The photosynthesis of plants is inseparable from the presence of chlorophyll. The determination of chlorophyll content is an important aspect of determining the photosynthetic characteristics of plants, and is also a relatively traditional measurement. Chlorophyll is insoluble in water but soluble in organic solvents such as alcohol and acetone. In the past, chlorophyll was first extracted and its content was measured by spectrophotometry. Although the chlorophyll extraction assay is simple, straightforward, and accurate, it is also obviously restrictive: it is destructive and very time consuming (especially in physioecological studies where the number of samples is often large). Thus, the recently produced and used chlorophyll meter provides an excellent opportunity to estimate chlorophyll levels rapidly and non-destructively.
The SPAD value is a relative chlorophyll content reading (reading range 0 to 9919), also known as greenness. Quantitatively describe the greenness of the blade by the magnitude of the value. Simply add the meter to the leaf tissue during the measurement and the SPAD value can be obtained within 2 seconds. The measured SPAD value (greenness) is positively correlated with the leaf chlorophyll content, which provides a reliable estimate of the chlorophyll level in field measurements . The chlorophyll meter is easy to carry, the measurement is simple and rapid, and the chlorophyll meter measurement is in-situ. It is measured under the condition that the leaves are not disturbed, and the plant leaves are not damaged. Therefore, continuous tracking measurement can be carried out to continue the related research. The conditions have been created to make this portable, non-destructive chlorophyll meter an irreplaceable advantage in some cases, especially in field experiments, and is a rapid test method worthy of promotion.
Therefore, using chlorophyll meter to determine the chlorophyll content of broad-leaved species is completely feasible. Because the chlorophyll meter is easy to carry and the measurement is simple and rapid, it can replace the direct determination of chlorophyll content in the field. In particular, this method does not damage the leaves. Therefore, continuous dynamic measurement can be performed, and in some special experimental requirements, Its irreplaceable advantages should be actively promoted in forestry research, especially with the increase in the application of remote sensing technology, there will be a large space for application of the chlorophyll meter SPAD-502 for determining the chlorophyll content of ground plants. Of course, like all research methods, it also has certain limitations. For example, the chlorophyll meter can only be used for hardwood species and it cannot be used for conifer species. In addition, it should be noted that in use, due to its small range of measurement, and the reaction is very sensitive, if you want to represent the green level of the entire blade, we must measure the different parts of the blade and then take the average.
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