The explants were inoculated into the culture medium of the tissue culture room and MS was used as the basic medium. The mitogen was used for the cross-combination test. Each combination was inoculated with 20 bottles. The growth was repeated 3 times and the proliferation was screened. The most suitable culture formula for the stage. The above medium added sucrose 30 g/L, carrageenan 6.5 g/L, pH 5.6, temperature 25°C, light intensity 2000 to 3000 lx, and light 12 h/d.
The key to early stage test in tissue culture chambers lies in the choice of exosomes. The trials collected three parts of young trees of silverwood (apical buds, middle axillary buds, and lower axillary buds) for initial induction screening, sterilized with 0.1% HgCl2, and selected the sterilization time according to the degree of lignification of the materials, and selected a low contamination rate. The best part of the buds and buds of early explants. The selection of axillary buds in the middle to make explants in tissue culture chambers is appropriate. Silverwood sterile lines can be established in the short term to save time and labor for future explants.
According to the experiments in tissue culture chambers, the budding time of the terminal buds is the shortest. The short exposure time of the top buds in the air is very short, the bacteria accumulation is minimal, the pollution after the treatment is minimal, and the end buds are too tender and sterilized. The process is difficult to control and cause the bud to be easily injured, which directly affects the induction effect of the terminal bud. From the tissue culture room, it was found that the axillary buds in the middle and lower parts were highly contaminated. Due to the deposition of meristems below the terminal buds, the strength of the material determines the length of induction time, the ability of the middle buds to produce a strong ability, and the axillary buds induced. sturdy.
Rope drum is one of the most important accessories in lifting equipment. It is responsible for winding the wire rope, carrying the tension and pressure of the lifting weight, and lifting and carrying the heavy object with the cooperation of other related crane structures.
In DGCRANE, there are mainly two type rope drum: Casting iron rope drum, Steel plate rope drum. (Some times, for the small diameter drum, we use the seamless steel tube). Generally speaking, the casting iron has self-lubrication property and is more comfortable for the wire rope. While, the plate rope drum is more suitable for big diameter rope drum. For big diameter drum, casting forming is more complex and the big weight is also another problem.
Which type is more suitabe for you is depends on your working situation. Of course, our engineers is always ready for you when you encounted with this question. In export, due to the different specification, a custom design rope drum is the most common thing. Now, this is the production capability that we can offer.
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